The only reason I could think of for it to be more complex is if she had ambiguous genitalia, then at that point I could see further tests, but for the vast majority of people, a simple look in the pants would be enough to confirm male or female.
Ambiguous genitalia? A vagenis?
http://www.nlm.nih.gov/MEDLINEPLUS/ency/article/003269.htm"Ambiguous genitalia is a birth defect where the outer genitals do not have the typical appearance of either a boy or a girl."
If the process that causes this fetal tissue to become "male" or "female" is disrupted, ambiguous genitalia can develop. This genitalia makes it difficult to classify the infant as male or female. The extent of the ambiguity varies. In very rare instances, the physical appearance may be fully developed as the opposite of the genetic sex. For example, a genetic male may have developed the appearance of a normal female.
Typically, ambiguous genitalia in genetic females (babies with two X chromosomes) has the following features:
An enlarged clitoris that has the appearance of a small penis.
The urethral opening (where urine comes out) can be anywhere along, above, or below the surface of the clitoris.
The labia may be fused, resembling a scrotum.
The infant may be thought to be a male with undescended testicles.
Sometimes a lump of tissue is felt within the fused labia, further making it look like a scrotum with testicles.
In a genetic male (one X and one Y chromosome), ambiguous genitalia typically include the following features:
A small penis (less than 2-3 centimeters or 0.8-1.2 inches) that resemble an enlarged clitoris (the clitoris of a newborn female is normally somewhat enlarged at birth).
The urethral opening may be anywhere along, above, or below the penis; it can be placed as low as on the peritoneum, further making the infant appear to be female.
There may be a small scrotum with any degree of separation, resembling labia.
Undescended testicles commonly accompany ambiguous genitalia.
Ambiguous genitalia is usually not life threatening (see Causes section for exceptions ), but can create social problems for the child and the family. For this reason, a team of experienced specialists, including neonatologists, geneticists, endocrinologists, and psychiatrists or social workers will be involved.